Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A high-density rice genetic linkage map with 2275 markers using a single F2 population

Y Harushima1, M Yano, A Shomura

  • 1Rice Genome Research Program, National Institute of Agrobiological Resources/Institute of Society for Techno-Innovation of Agriculture, Forestry, and Fisheries, Tsukuba, Ibaraki, Japan.

Genetics
|February 25, 1998
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dynamics of M1 macrophages in oral mucosal lesions during the development of acute graft-versus-host disease in rats.

Clinical and experimental immunology·2017
Same author

Mevalonates restore zoledronic acid-induced osteoclastogenesis inhibition.

Journal of dental research·2014
Same author

Genetic Evaluation and Utilization (GEU) program : The rice improvement program of the International Rice Research Institute.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Genetic analysis of brown planthopper resistance in twenty varieties of rice, Oryza saliva L.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Genetic analysis of bacterial blight resistance in seventy-four cultivars of rice, Oryza sativa L.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013
Same author

Heterosis breeding in rice (Oryza sativa L.).

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013

Researchers created the most detailed genetic map for rice (Oryza sativa L.) using expressed sequence tag (EST) markers. This high-resolution map aids in understanding meiotic recombination and gene locations in plants.

Area of Science:

  • Genetics
  • Plant Biology
  • Genomics

Background:

  • Developing high-resolution genetic maps is crucial for understanding plant genomes.
  • Rice (Oryza sativa L.) is a model organism for cereal crop research.
  • Previous genetic maps lacked the detail to fully characterize meiotic recombination.

Purpose of the Study:

  • To construct the most detailed genetic map of rice to date.
  • To identify centromere locations and analyze meiotic recombination.
  • To utilize expressed sequence tag (EST) markers for comprehensive genome coverage.

Main Methods:

  • A genetic map was constructed using 186 F2 plants from a cross between Nipponbare (japonica) and Kasalath (indica) rice varieties.
  • Over 2275 DNA markers, primarily ESTs, were employed to cover 1521.6 cM of the rice genome.

Related Experiment Videos

  • Centromere positions were determined using dosage analysis of trisomic plants and DNA markers.

    • Main Results:

    • A 2275-marker genetic map of rice was successfully constructed, representing the most detailed map available for any plant species.
    • 1455 loci were mapped using ESTs, with 615 showing similarity to known genes.
    • Centromere locations were identified on all 12 rice linkage groups, and meiotic recombination patterns were characterized.

    Conclusions:

    • The high-resolution rice genetic map provides an invaluable resource for plant genomics and genetic studies.
    • The study characterized meiotic recombination across the entire genome, revealing positive interference.
    • The findings enhance our understanding of genome structure and recombination mechanisms in rice.