Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Solid-phase, magnetic particle radioimmunoassay

L Nye, G C Forrest, H Greewood

    Clinica Chimica Acta; International Journal of Clinical Chemistry
    |June 15, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Assessment of dermal sensitization by nickel salts in a novel humanized TLR-4 mouse model.

    Journal of immunotoxicology·2024
    Same author

    Mycobacteroides abscessus outbreak and mitigation in a cardiothoracic transplant population: the problem with tap water.

    The Journal of hospital infection·2024
    Same author

    Iron limitation induces motility in uropathogenic <i>E. coli</i> CFT073 partially through action of LpdA.

    mBio·2024
    Same author

    Altered motility in response to iron-limitation is regulated by <i>lpdA</i> in uropathogenic <i>E. coli</i> CFT073.

    bioRxiv : the preprint server for biology·2023
    Same author

    Examination of the exposome in an animal model: The impact of high fat diet and rat strain on local and systemic immune markers following occupational welding fume exposure.

    Toxicology and applied pharmacology·2023
    Same author

    The Calculated Continuous Emission of a LTE Hydrogen Plasma.

    Journal of research of the National Bureau of Standards. Section A, Physics and chemistry·2021
    Same journal

    Reference intervals for venous blood gas measurement in a healthy Chinese population.

    Clinica chimica acta; international journal of clinical chemistry·2026
    Same journal

    Multiplex methylation marker analysis for ctDNA detection in liquid biopsies from anal cancer patients: an HPV-independent approach.

    Clinica chimica acta; international journal of clinical chemistry·2026
    Same journal

    Development and validation of patient-based exponentially weighted moving average quality control models for three antipsychotic drugs and their metabolites by LC-MS/MS.

    Clinica chimica acta; international journal of clinical chemistry·2026
    Same journal

    Comparing conventional correction formulas and machine learning-based prediction of ionized calcium.

    Clinica chimica acta; international journal of clinical chemistry·2026
    Same journal

    Micro- and nanoplastics as emerging clinical analytes: analytical validation, interpretive uncertainty, and laboratory actionability in human specimens.

    Clinica chimica acta; international journal of clinical chemistry·2026
    Same journal

    Performance of Free Light Chain reagents in the Dutch External Quality Assessment programme over the past 14 years.

    Clinica chimica acta; international journal of clinical chemistry·2026
    See all related articles

    A novel solid-phase radioimmunoassay uses magnetic iron oxide particles for faster, simpler immunoassays. This magnetic separation method eliminates centrifugation, improving efficiency for hormone and drug testing.

    Area of Science:

    • Biochemistry
    • Immunochemistry
    • Materials Science

    Background:

    • Conventional solid-phase immunoassays often require complex separation steps like centrifugation.
    • These steps can be time-consuming and labor-intensive, potentially affecting assay efficiency and reproducibility.

    Purpose of the Study:

    • To develop a simplified and efficient solid-phase radioimmunoassay system.
    • To utilize magnetic separation for antibody-bound and free fraction separation.
    • To demonstrate the system's applicability across various analytes.

    Main Methods:

    • Antibodies were covalently linked to polymer-coated iron oxide particles.
    • An electromagnet was used for particle mixing and magnetic separation.
    • Assays for thyroxine, human placental lactogen, and digoxin were established.

    Related Experiment Videos

    Main Results:

    • The magnetic separation system effectively mixed particles and separated antibody-bound/free fractions.
    • This eliminated the need for centrifugation and vertical rotation.
    • Thyroxine assay results closely correlated with a routine liquid-phase radioimmunoassay.

    Conclusions:

    • The developed magnetic solid-phase radioimmunoassay system offers a simplified and efficient alternative.
    • The system is versatile and applicable to various immunoassay targets.
    • Further studies explored the use of a second antibody linked to iron oxide particles.