Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A cryopreservation method for Pasteurella multocida from wetland samples

M K Moore1, D J Shadduck, D R Goldberg

  • 1U.S. Geological Survey, Biological Resources Division, National Wildlife Health Center, Madison, Wisconsin 53711, USA.

Journal of Wildlife Diseases
|February 26, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Trichinella murrelli in scavenging mammals from south-central Wisconsin, USA.

Journal of wildlife diseases·2008
Same author

Onychomycosis.

Revista iberoamericana de micologia·2008
Same author

Structure-based design and subsequent optimization of 2-tolyl-(1,2,3-triazol-1-yl-4-carboxamide) inhibitors of p38 MAP kinase.

Bioorganic & medicinal chemistry letters·2008
Same author

Characterization of Toxoplasma gondii from raccoons (Procyon lotor), coyotes (Canis latrans), and striped skunks (Mephitis mephitis) in Wisconsin identified several atypical genotypes.

The Journal of parasitology·2008
Same author

Modest genetic differentiation among North American populations of Sarcocystis neurona may reflect expansion in its geographic range.

Veterinary parasitology·2008
Same author

Pyrazinoindolone inhibitors of MAPKAP-K2.

Bioorganic & medicinal chemistry letters·2008
Same journal

Marked Decline in Bearded Pig (Sus barbatus) Detections in Sabah, Malaysia, during the African Swine Fever Outbreak, 2021.

Journal of wildlife diseases·2026
Same journal

Mucormycosis in a Platypus (Ornithorhynchus anatinus) on the Australian Mainland.

Journal of wildlife diseases·2026
Same journal

Evaluation of Pronghorn (Antilocapra americana) Chemical Immobilization in a Captive Research Facility in Wyoming, USA (2021-24).

Journal of wildlife diseases·2026
Same journal

Blood Parasites of the Phylum Apicomplexa and Hematological Values in European Pond Turtles (Emys orbicularis) from Lublin Voivodeship, Eastern Poland.

Journal of wildlife diseases·2026
Same journal

Profiling of the Guadalupe Fur Seal (Arctocephalus philippii townsendii) Fecal Bacteriome in Relation to Alopecic Syndrome.

Journal of wildlife diseases·2026
Same journal

Comparison of Detection Rates of Toxoplasma gondii among Five Host Tissues and Two Primer Sets in Three Bird Species.

Journal of wildlife diseases·2026
See all related articles

A new cryopreservation method effectively isolates Pasteurella multocida from wetland samples. This technique preserves samples for later analysis, offering a reliable alternative to traditional methods.

Area of Science:

  • Environmental Microbiology
  • Bacteriology

Background:

  • Pasteurella multocida is a significant pathogen found in various environments.
  • Accurate detection of P. multocida in environmental samples like wetlands is crucial for disease surveillance.
  • Existing isolation methods may face challenges with sample preservation and transport.

Purpose of the Study:

  • To develop and validate a cryopreservation method for Pasteurella multocida detection in wetland samples.
  • To improve isolation techniques for enhanced P. multocida recovery.

Main Methods:

  • Wetland water samples were collected and diluted in 10% dimethyl sulfoxide (DMSO).
  • Samples were frozen at -180°C using a liquid nitrogen vapor shipper for transport.
  • Frozen samples were thawed and processed using Pasteurella multocida selective broth (PMSB) for isolation.

Related Experiment Videos

Main Results:

  • The cryopreservation method allowed consistent isolation of P. multocida, ranging from 2 to 18 organisms/ml.
  • The developed method demonstrated favorable comparison with the standard mouse inoculation technique.
  • Sample preservation was achieved, enabling laboratory processing at a later time.

Conclusions:

  • Cryopreservation in DMSO is an effective strategy for preserving P. multocida in wetland samples.
  • Improved isolation techniques combined with cryopreservation enhance P. multocida detection capabilities.
  • This method offers a viable alternative for sample handling in environmental microbiology studies.