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Typing of Candida albicans by use of a combined PCR/hybridization assay
1Klinikums Grosshadern, Ludwig-Maximilians-Universität München, FR Germany.
Mycoses
|February 26, 1998
Abstract:
A modified genotyping assay for Candida albicans based on the RAPD-PCR (random amplification of polymorphic DNA) is described. Following capillary blot of RAPD-PCR products onto a nylon membrane, hybridization with a PCR-generated gene probe resulted in heterogeneous band patterns. By this method the discriminatory power of RAPD-PCR is enhanced with satisfactory reproducibility and reduced interference.