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Related Experiment Videos

Submicrovolt full-field cone electroretinograms: artifacts and reproducibility

D G Birch1, M A Sandberg

  • 1Retina Foundation of the Southwest, Dallas, TX 75231, USA.

Documenta Ophthalmologica. Advances in Ophthalmology
|January 1, 1996
PubMed
Summary

This study evaluated submicrovolt electroretinograms, finding that bipolar lenses effectively minimize artifacts. This technique offers reliable test-retest variation for patients with retinal diseases.

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Area of Science:

  • Ophthalmology
  • Neuroscience
  • Medical Engineering

Background:

  • Submicrovolt electroretinograms (ERGs) are crucial for diagnosing retinal diseases.
  • Electrical and photoelectric artifacts from xenon-flash stimulators can contaminate low-amplitude ERGs.
  • Quantifying and minimizing these artifacts is essential for clinical utility.

Purpose of the Study:

  • To assess the clinical utility of submicrovolt, full-field, 30-Hz (cone) electroretinograms.
  • To quantify artifact contamination and test-retest variation in patients with retinitis pigmentosa.
  • To evaluate artifact reduction techniques, particularly the use of bipolar lenses.

Main Methods:

  • Artifacts from xenon-flash stimulators were quantified using four common electrode types in saline.

Related Experiment Videos

  • Electroretinogram recordings were performed on patients with advanced retinitis pigmentosa and congenital rod monochromatism.
  • Narrow-band-filtered responses were recorded twice within 6 weeks from patients with amplitudes < 1 microV to assess test-retest variation.

    • Main Results:

    • Artifacts varied by electrode type, comprising electrical and photoelectric components.
    • Bipolar lenses effectively eliminated artifacts in ERG recordings from patients.
    • The threshold criteria for significant amplitude change (p < 0.05) were approximately 0.25 log unit for test-retest variation.

    Conclusions:

    • Submicrovolt full-field 30-Hz electroretinograms are clinically useful when artifacts are minimized.
    • Bipolar lenses are highly effective in eliminating electrical and photoelectric artifacts.
    • The described methods provide reliable test-retest variation assessment for low-amplitude ERGs in retinal disease patients.