Antigenicity of deoxyadenosine 5'-monophosphate cross-linked with polyamine

Deoxyadenosine 5'-monophosphate was cross-linked to spermine using glutaraldehyde as cross-linking reagent. The complex thus obtained was characterized by UV absorption spectroscopy, size-exclusion chromatography and CD. The complex had a molecular mass of around 10,200 Da. Antibodies were raised against this complex in rabbits and the titre obtained was found to be > 1:3200. The fine specificity of the raised antibodies was checked by competition ELISA. The complex also bound to naturally occurring anti-DNA autoantibodies, suggesting a possible role of the complex in the pathogenesis of systemic lupus erythematosus.