Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bone remodeling with resorbable bioactive glass and hydroxyapatite

Y Shimizu1, H Sugawara, T Furusawa

  • 1Department of Oral Bacteriology, Tohoku University, School of Dentistry, Sendai, Japan.

Implant Dentistry
|January 1, 1997
PubMed
Summary

This study examined how two types of resorbable materials—bioactive glass and hydroxyapatite—affect bone remodeling in cell cultures. Researchers found that both materials supported the growth of periodontal cells and osteoblasts without harmful effects. Bioactive glass activated cells involved in bone formation better than hydroxyapatite and reduced the formation of cells that break down bone. The materials did not affect lymphocytes or monocytes negatively. These findings suggest that bioactive glass could be more useful for bone regeneration than hydroxyapatite. The study highlights the potential of these materials in developing biocompatible implants for bone tissue engineering.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characteristics of the magnetic resonance imaging findings of cervical gastric-type adenocarcinoma.

Clinical radiology·2024
Same author

Faster acquisition of magnetic resonance imaging sequences of the knee via deep learning reconstruction: a volunteer study.

Clinical radiology·2024
Same author

Quasilinear quantum magnetoresistance in pressure-induced nonsymmorphic superconductor chromium arsenide.

Nature communications·2017
Same author

Superconductivity and magnetic fluctuations developing in the vicinity of strong first-order magnetic transition in CrAs.

Journal of physics. Condensed matter : an Institute of Physics journal·2017
Same author

Divalent cations enhance fluoride binding to Streptococcus mutans and Streptococcus sanguinis cells and subsequently inhibit bacterial acid production.

Caries research·2012
Same author

Hypermethylation of serotonin transporter gene in bipolar disorder detected by epigenome analysis of discordant monozygotic twins.

Translational psychiatry·2012

Area of Science:

  • Biomedical materials in regenerative medicine
  • Cellular responses to biomaterials in dentistry
  • Bone remodeling mechanisms in tissue engineering

Background:

Current research explores the effects of biomaterials on cellular behavior during bone remodeling. Prior studies have shown that certain materials can influence osteoblast and osteoclast activity. However, the specific impact of resorbable bioactive glass and hydroxyapatite on periodontal cells remains unclear. This uncertainty drives the need for controlled in vitro studies. No prior work had resolved whether these materials could support bone formation without harmful effects. The lack of detailed data on cell-material interactions creates a gap in the field. Understanding how these materials affect lymphocytes and monocytes is also important. This gap motivated experiments to assess the biocompatibility and osteoinductive potential of resorbable bioactive glass and hydroxyapatite.

Purpose Of The Study:

The aim of this study was to evaluate how resorbable bioactive glass and hydroxyapatite affect periodontal ligament cells and osteoblasts. Researchers focused on whether these materials could support bone formation without toxic effects. The specific problem addressed was the need for biocompatible materials that promote bone regeneration. Motivation came from the clinical demand for resorbable implants that integrate with surrounding tissues. The study also sought to determine if these materials influence osteoclast formation. A key question was whether these materials could activate alkaline phosphatase in cultured cells. Researchers wanted to compare the effects of bioactive glass and hydroxyapatite on cell adhesion and proliferation. The ultimate goal was to identify materials that support bone remodeling without adverse effects.

Keywords:
resorbable bioactive glassbone remodelingosteoblast activitybiomaterials in dentistry

Frequently Asked Questions

According to the authors, resorbable bioactive glass activates cells necessary for bone formation and suppresses osteoclast formation.

The study found that both bioactive glass and hydroxyapatite promoted alkaline phosphatase activation in cultured cells.

The researchers propose that suppressing osteoclasts may help reduce excessive bone resorption during remodeling.

The number of adherent and nonadherent cells remained unchanged compared to control groups.

Related Experiment Videos

Main Methods:

The study used in vitro cell culture techniques to assess the effects of resorbable bioactive glass and hydroxyapatite. Researchers cultured periodontal ligament cells and osteoblasts on the surfaces of these materials. They measured alkaline phosphatase activity as an indicator of osteoblast function. Peripheral blood lymphocytes and monocytes were also exposed to the materials. Cell adhesion and proliferation were analyzed using standard cell culture methods. Researchers compared the number of adherent and nonadherent cells to control groups. The presence of osteoclasts was assessed to determine the impact on bone resorption. The study design allowed for direct comparison of the two materials' biological effects.

Main Results:

Resorbable bioactive glass and hydroxyapatite did not inhibit the growth of periodontal ligament cells or osteoblasts. Both materials supported the activation of alkaline phosphatase around their surfaces. When cultured with peripheral blood lymphocytes and monocytes, the materials did not alter cell adhesion or proliferation. Bioactive glass activated cells necessary for bone formation more effectively than hydroxyapatite. The material also suppressed the formation of osteoclasts involved in bone resorption. These findings suggest bioactive glass may support bone remodeling better than hydroxyapatite. No harmful effects were observed on periodontal cells or lymphocytes/monocytes. The results indicate these materials are biocompatible and suitable for bone regeneration.

Conclusions:

The study found that resorbable bioactive glass and hydroxyapatite do not harm periodontal cells or lymphocytes/monocytes. Bioactive glass activated cells involved in bone formation better than hydroxyapatite. The material also reduced the formation of osteoclasts, which may aid in bone remodeling. These findings suggest bioactive glass could be more effective for bone regeneration. The materials did not inhibit cell growth or alkaline phosphatase activity. Researchers observed no adverse effects on cell adhesion or proliferation. The results support the use of these materials in clinical applications. The study highlights the potential of resorbable bioactive glass for bone tissue engineering.

Failed At:

2026-07-14T07:37:19.719047+00:00

The study suggests this enzyme activity indicates osteoblast function and potential for bone formation.

The authors propose that resorbable bioactive glass may be more effective for bone remodeling than hydroxyapatite.