Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A poststaining fixation technique for acridine orange: quantitative aspects

M A Cambier, L L Wheeless, S F Patten

    Analytical and Quantitative Cytology
    |March 1, 1979
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Chromosome 9 monosomy by fluorescence in situ hybridization of bladder irrigation specimens is predictive of tumor recurrence.

    The Journal of urology·1999
    Same author

    Strongyloides stercoralis infection presenting as generalized prurigo nodularis and lichen simplex chronicus.

    Journal of the American Academy of Dermatology·1999
    Same author

    DNA cytometry and chromosome 9 aberrations by fluorescence in situ hybridization of irrigation specimens from bladder cancer patients.

    Urology·1998
    Same author

    Biomarker study of primary nonmetastatic versus metastatic invasive bladder cancer. National Cancer Institute Bladder Tumor Marker Network.

    Clinical cancer research : an official journal of the American Association for Cancer Research·1998
    Same author

    The AutoPap 300 QC System multicenter clinical trials for use in quality control rescreening of cervical smears: II. Prospective and archival sensitivity studies.

    Cancer·1998
    Same author

    The AutoPap 300 QC System multicenter clinical trials for use in quality control rescreening of cervical smears: I. A prospective intended use study.

    Cancer·1998
    Same journal

    Quantitative analysis of heterogeneity of estrogen binding in human breast tumor specimens.

    Analytical and quantitative cytology·1984
    Same journal

    Analysis of number, size and distribution patterns of lipid vacuoles in benign and malignant mesothelial cells.

    Analytical and quantitative cytology·1984
    Same journal

    Automated measurement of acid phosphatase reaction product in human kidneys treated with aminoglycoside antibiotics.

    Analytical and quantitative cytology·1984
    Same journal

    The use of cationic polyelectrolytes in the preparation of cell monolayers for automated cell scanning and diagnostic cytopathology.

    Analytical and quantitative cytology·1984
    Same journal

    Simulation model for gynecologic specimen classification in a high-resolution prescreening system.

    Analytical and quantitative cytology·1984
    Same journal

    Stabilized binary hierarchic classifier in cytopathologic diagnosis.

    Analytical and quantitative cytology·1984
    See all related articles

    A new poststaining fixation technique for acridine orange staining improves cell storage and fluorescence consistency. This method enhances analytic cytology and automated screening potential for cytologic specimens.

    Area of Science:

    • Cytopathology
    • Analytical Cytology
    • Biomedical Imaging

    Background:

    • Acridine orange staining is crucial for cytologic specimen analysis.
    • Conventional staining methods face challenges with cell storage and fluorescence stability.
    • Need for improved techniques in analytic cytology and automated screening.

    Purpose of the Study:

    • To develop and evaluate a poststaining fixation technique for acridine orange staining.
    • To assess its suitability for routine analytic cytology.
    • To explore its potential for automated screening equipment.

    Main Methods:

    • Development of a novel poststaining fixation protocol for acridine orange.
    • Quantitative evaluation of stained cytologic specimens.

    Related Experiment Videos

  • Comparison with conventional acridine orange staining methods.

    • Main Results:

    • Stained cells can be stored for months without altering morphology or fluorescence.
    • Minimized diffusion of acridine orange stain out of cells.
    • Reduced dependence of fluorescence on cell distribution/concentration.

    Conclusions:

    • The poststaining fixation technique offers significant advantages for acridine orange staining.
    • It is suitable for routine analytic cytology and shows promise for automated screening.
    • This technique enhances the reliability and utility of cytologic specimen analysis.