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Related Experiment Videos

Keratocyte apoptosis after corneal surgery

M C Helena1, F Baerveldt, W J Kim

  • 1Eye Institute, Cleveland Clinic Foundation, Ohio 44195, USA.

Investigative Ophthalmology & Visual Science
|March 7, 1998
PubMed
Summary

Keratocyte apoptosis, or programmed cell death, is a key factor in corneal wound healing after refractive surgery. Transepithelial PRK showed the lowest apoptosis levels, suggesting its potential for treating myopia.

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Area of Science:

  • Ophthalmology
  • Cell Biology
  • Regenerative Medicine

Background:

  • Programmed cell death (apoptosis) is crucial for tissue homeostasis and repair.
  • Keratocyte apoptosis is hypothesized to initiate wound healing post-refractive surgery.

Purpose of the Study:

  • To evaluate keratocyte apoptosis after various refractive surgical procedures.
  • To compare apoptosis levels induced by traditional epithelial scrape-photorefractive keratectomy (PRK), transepithelial PRK, microkeratome procedures, and laser-assisted in situ keratomileusis (LASIK).

Main Methods:

  • Rabbit corneas underwent refractive surgical procedures or components.
  • Keratocyte apoptosis was assessed using the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay.
  • Cellular morphology was examined via electron microscopy.

Main Results:

  • All procedures induced variable keratocyte apoptosis.
  • Transepithelial PRK exhibited the lowest central corneal apoptosis levels.
  • Apoptosis was superficial (50-75 microns) after PRK, but deeper around the lamellar cut in LASIK.

Conclusions:

  • Qualitative and quantitative differences in keratocyte apoptosis exist between LASIK and PRK subtypes.
  • Epithelial injury significantly influences keratocyte apoptosis.
  • Transepithelial PRK's low apoptosis suggests utility for high myopia and retreatment.

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