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Choroid plexus histidine transport

J Xiang1, R L Fowkes, R F Keep

  • 1Department of Surgery (Neurosurgery), University of Michigan, R5605 Kresge I, Ann Arbor, Michigan, MI 48109-0532, USA.

Brain Research
|May 2, 1998
PubMed
Summary
This summary is machine-generated.

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System-N transport is crucial for l-histidine uptake in rat choroid plexus, with Na+-dependent transport being pH-sensitive. Pathophysiological conditions like low pH and high l-glutamine impact histidine transport.

Area of Science:

  • Neuroscience
  • Biochemistry
  • Physiology

Background:

  • System-N transporters are vital for amino acid uptake, including l-glutamine, in the rat choroid plexus.
  • The role of System-N in l-histidine transport and its modulation by pH and l-glutamine remains unclear.

Purpose of the Study:

  • To determine the role of System-N in l-histidine transport across the isolated rat choroid plexus.
  • To investigate the impact of altered pH and l-glutamine levels on System-N mediated l-histidine transport.

Main Methods:

  • Utilized isolated rat choroid plexus to measure l-[3H]histidine uptake.
  • Differentiated transport mechanisms (Na+-dependent vs. Na+-independent) using specific inhibitors and varying substrate concentrations.
  • Assessed the effects of pH changes and elevated l-glutamine levels on histidine transport.

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Main Results:

  • l-Histidine uptake is mediated by both Na+-independent (System-L) and Na+-dependent (System-N) transporters.
  • Na+-dependent transport exhibited Michaelis-Menten kinetics with a Km of 0.2 mM and Vmax of 1.4 nmol/mg/min, accounting for 30% of uptake.
  • Reduced pH significantly inhibited Na+-dependent transport, while increased l-glutamine decreased uptake via both systems.

Conclusions:

  • System-N plays a significant role in l-histidine uptake at the choroid plexus.
  • System-N mediated l-histidine transport is sensitive to pH changes, unlike in neurons.
  • Pathophysiological conditions involving altered pH and l-glutamine concentrations can impair l-histidine transport at the choroid plexus.