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[Comparison of thrombocyte concentrates from buffy coats after 3 and 16 hours of storage]
H Häberle1, T Schulzki, V Kretschmer
1Abteilung für Transfusionsmedizin und Gerinnungsphysiologie, Universität Marburg, Deutschland.
Insights
Storing buffy coat (BC) longer than 3 hours before preparing platelet concentrates (PC) may reduce platelet quality. Longer BC storage impacted morphology, pH, and lactate, with some PC becoming unusable. Quality control is crucial for PC from extended BC storage.
Area of Science:
- Transfusion Medicine
- Hematology
- Blood Component Preparation
Background:
- Extended storage of buffy coat (BC) may negatively impact platelet function due to granulocyte proteases.
- Platelet concentrates (PC) are critical for managing bleeding disorders.
Purpose of the Study:
- To investigate the effect of buffy coat resting time on platelet concentrate quality.
- To compare PC quality derived from BC stored for 3 hours versus 16 hours.
Main Methods:
- Platelet concentrates were prepared from buffy coats rested for either 3 hours (n=18) or 16 hours (n=41).
- Quality parameters including morphology score, pH, and lactate were assessed.
- Differences were monitored over a 5-day storage period.
Main Results:
- Significant differences in morphology score, pH, and lactate favored PC from 3-hour-BC.
- These quality differences diminished over the 5-day storage period.
- Unacceptable quality PC (n=4) were exclusively derived from 16-hour-BC.
Conclusions:
- Shortening buffy coat resting time to 3 hours before processing improves initial platelet concentrate quality.
- Extended buffy coat storage (≥16 hours) can lead to unacceptable platelet concentrate quality.
- Adequate quality control is essential for platelet concentrates derived from buffy coats stored for over 4 hours.
Abstract:
Since extended storage of the buffy coat (BC) might be disadvantageous for the platelet function because of granulocyte proteases we investigated platelet concentrates (PC) prepared from BC which rested either for 3 h (n = 18) or 16 h (n = 41) before further preparation. We found significant differences especially in the morphology score, pH and lactate in favour of the PC from 3-hour-BC. The differences decreased during the 5-day storage. PC of unacceptable quality (n = 4) only derived from 16-hour-BC. Therefore, the use of PC from BC stored for more than 4 h at least requires an adequate quality control before delivery.