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Generation and characterization of heritable reciprocal translocations in mice

L Stubbs1, E A Carver, N L Cacheiro

  • 1Life Sciences Division, Oak Ridge National Laboratory, Tennessee 37831-8077, USA.

Methods (San Diego, Calif.)
|March 21, 1998
PubMed

Insights

Reciprocal translocations in mice are induced via radiation or chemicals. These genetic tools efficiently generate mutations for studying gene function and developmental consequences of partial aneuploidy in mammals.

Area of Science:

  • Genetics
  • Developmental Biology
  • Cancer Research

Background:

  • Reciprocal translocations are vital for gene localization in human diseases.
  • Heritable translocations are rare in humans but inducible in mice.
  • Translocations aid in understanding genetic disorders and cancer.

Purpose of the Study:

  • To summarize mutagenesis schemes for high-frequency translocation induction in mice.
  • To review the application of translocation mutations in genetic and developmental studies.
  • To describe preliminary findings from a collection of translocation mutants.

Main Methods:

  • Induction of translocations in male mouse germ cells using radiation and chemicals.
  • Analysis of progeny from treated males for translocation frequency.
  • Phenotypic characterization of generated translocation mutants.

Main Results:

  • Several mutagenesis protocols efficiently generate reciprocal translocations in mice.
  • Translocation mutants provide tools for analyzing partial aneuploidy consequences.
  • Mutations identified display dominant or recessive phenotypes due to gene disruption.

Conclusions:

  • Efficient generation of easily mapped mouse mutations using mutagenesis protocols.
  • Reciprocal translocations are invaluable for studying mammalian gene function.
  • These genetic tools facilitate research into human cancers and hereditary diseases.

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