Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Vesicles on strings: morphological evidence for processive transport within the Golgi stack

L Orci1, A Perrelet, J E Rothman

  • 1Department of Morphology, University of Geneva Medical School, 1211 Geneva 4, Switzerland.

Proceedings of the National Academy of Sciences of the United States of America
|April 16, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The oxygen concentrator: an appropriate technology for treating hypoxaemic children in developing countries.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2004
Same author

A t-SNARE of the endocytic pathway must be activated for fusion.

The Journal of cell biology·2001
Same author

Molecular mass, stoichiometry, and assembly of 20 S particles.

The Journal of biological chemistry·2001
Same author

The debate about transport in the Golgi--two sides of the same coin?

Cell·2000
Same author

The use of pHluorins for optical measurements of presynaptic activity.

Biophysical journal·2000
Same author

Compartmental specificity of cellular membrane fusion encoded in SNARE proteins.

Nature·2000
Same journal

In This Issue.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Long-term cultural continuity across the Neanderthal-modern human sequence at Üçağızlı II Cave, northern Levant.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Dolphins use names to remember whom to avoid.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Retraction for Shaked and Frenkel, Curiouser and curiouser: Meningeal lymphoid structures in the aging brain.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Small but mighty: The outsized role of small water bodies in the global carbon cycle.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same journal

Functional traits produce conditional outcomes in different community contexts.

Proceedings of the National Academy of Sciences of the United States of America·2026
See all related articles

Golgi transport vesicles stay near their origin, suggesting a processive transport model. "String theory" proposes proteins tether vesicles to adjacent Golgi cisternae, limiting diffusion.

Area of Science:

  • Cell Biology
  • Molecular Transport
  • Golgi Apparatus Dynamics

Background:

  • The Golgi apparatus is crucial for protein modification and sorting.
  • Understanding intra-Golgi transport mechanisms is key to cellular function.
  • Previous models did not fully explain vesicle localization within the Golgi stack.

Purpose of the Study:

  • To investigate the localization and movement of transport vesicles within the Golgi stack.
  • To propose a novel mechanism for processive intra-Golgi transport.

Main Methods:

  • Utilized freeze-fracture electron microscopy to analyze particle density in Golgi cisternae and vesicles.
  • Observed COPI-coated and uncoated transport vesicles in proximity to Golgi cisternae.

Related Experiment Videos

Main Results:

  • Cis-Golgi cisternae exhibit higher particle density than trans-cisternae.
  • Transport vesicles show particle concentrations similar to adjacent cisternae, indicating localized behavior.
  • Evidence suggests vesicles remain near their budding site, favoring transfer to adjacent cisternae.

Conclusions:

  • Transport vesicles are likely confined to specific regions within the Golgi stack.
  • A 'string theory' is proposed, involving fibrous Golgi-resident proteins (e.g., p115, GM130) tethering vesicles.
  • This tethering mechanism facilitates processive transport by limiting vesicle diffusion to adjacent cisternae.