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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: May 8, 2026

Flow Cytometry-based Purification of S. cerevisiae Zygotes
15:09

Flow Cytometry-based Purification of S. cerevisiae Zygotes

Published on: September 21, 2012

Flow cytometry and cell sorting for yeast viability assessment and cell selection

D Deere1, J Shen, G Vesey

  • 1Macquarie University Centre for Analytical Biotechnology, School of Biological Sciences, Macquarie University, NSW, Australia. ddeere@rna.bio.mq.edu.au

Yeast (Chichester, England)
|March 4, 1998
PubMed
Summary

Flow cytometry offers rapid yeast viability assessment, providing results in 20 minutes compared to traditional 36-hour colony counts. Propidium iodide (PI) and ChemChrome Y (CY) dyes accurately distinguish live and dead yeast cells.

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Last Updated: May 8, 2026

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Area of Science:

  • Microbiology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Traditional yeast viability assessment via colony counting is time-consuming (36 hours).
  • Rapid and accurate methods are crucial for industrial yeast cultures and high activity dried yeast (HADY).

Purpose of the Study:

  • To evaluate flow cytometry with fluorescent dyes for rapid yeast viability determination.
  • To compare the efficacy of various dyes (ChemChrome Y, oxonol, propidium iodide, Fungolight, rhodamine 123) for live/dead cell discrimination.

Main Methods:

  • Yeast suspensions were analyzed using flow cytometry after staining with fluorescent dyes.
  • Dye performance was assessed for total and viable cell densities in industrial yeast and HADY.
  • Cell sorting was performed after staining to confirm yeast viability post-analysis.

Main Results:

  • Flow cytometry provided results within 20 minutes, significantly faster than colony counts.
  • Propidium iodide (PI), oxonol (Ox), and ChemChrome Y (CY) demonstrated effective live/dead discrimination.
  • PI stained non-culturable cells, while CY stained culturable cells; PI and CY assays showed agreement in viability assessment.

Conclusions:

  • Flow cytometry with PI, Ox, or CY offers a rapid and accurate method for yeast viability testing.
  • These methods enable the physical selection of viable yeast cells from mixed populations.
  • PI and CY assays are reliable for assessing viability in various yeast preparations, with Ox showing differences in HADY.