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[Isolation frequency and biological characteristics of the multiple-antibiotic resistant Pseudomonas aeruginosa

M Okazaki1, T Onogawa, K Araki

  • 1Department of Clinical Laboratories, Kyorin University Hospital.

Kansenshogaku Zasshi. the Journal of the Japanese Association for Infectious Diseases
|March 4, 1998
PubMed

Insights

Multiple-antibiotic resistant Pseudomonas aeruginosa (MARPA) strains, predominantly serotype C, were identified in a hospital outbreak. These resistant strains showed reduced production of virulence factors compared to sensitive strains.

Area of Science:

  • Clinical Microbiology
  • Infectious Diseases
  • Epidemiology

Background:

  • Pseudomonas aeruginosa is an opportunistic pathogen frequently associated with hospital-acquired infections.
  • The emergence of multiple-antibiotic resistant Pseudomonas aeruginosa (MARPA) poses a significant threat to patient care.
  • Understanding the characteristics and epidemiology of MARPA is crucial for infection control.

Purpose of the Study:

  • To investigate the prevalence, serotypes, and virulence factor production of MARPA isolated from clinical specimens.
  • To compare the characteristics of MARPA with antibiotic-sensitive P. aeruginosa (ASPA).
  • To determine the epidemiological relationship of MARPA strains within a hospital setting.

Main Methods:

  • Isolation and identification of P. aeruginosa strains from clinical specimens.
  • Determination of antibiotic resistance profiles and serotyping.
  • Characterization of virulence factor production (pyocyanin, pyoverdin, hemolysin, elastase, caseinase).

Main Results:

  • MARPA accounted for 11.9% of P. aeruginosa isolates.
  • The majority of MARPA strains (88.9%) belonged to serotype C.
  • MARPA strains exhibited reduced production of key virulence factors compared to ASPA strains.

Conclusions:

  • The findings suggest that MARPA strains, particularly serotype C, may have spread from a common clone within the hospital.
  • The reduced virulence factor production in MARPA warrants further investigation into the mechanisms of resistance and pathogenicity.
  • These results highlight the importance of continuous surveillance and infection control measures against MARPA in healthcare settings.

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