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Senescence-induced RNases in tomato

A Lers¹, A Khalchitski, E Lomaniec

¹Department of Postharvest Science of Fresh Produce, Volcani Center, Bet Dagan, Israel.

Plant Molecular Biology

|March 4, 1998

Summary

Researchers identified two tomato RNase genes, LX and LE, involved in RNA breakdown during leaf senescence. LX is highly induced in late senescence, while LE may act as a plant defense protein during wounding.

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Area of Science:

Plant Molecular Biology
Biochemistry
Gene Expression Analysis

Background:

Leaf senescence involves macromolecule hydrolysis and redistribution.
Ribonucleases (RNases) play a role in nucleic acid catabolism during senescence.
Previous studies showed LX and LE RNase genes induced by phosphate starvation in tomato cell cultures.

Purpose of the Study:

To characterize RNases involved in nucleic acid catabolism during tomato leaf senescence.
To investigate the expression patterns and potential functions of LX and LE RNase genes in plants.

Main Methods:

PCR-based cloning to isolate senescence-induced RNase cDNA clones from tomato.
Northern blot analysis to detect transcript levels of LX and LE RNases.
Analysis of gene expression in response to ethylene and abscisic acid treatments, and wounding.

Main Results:

Two senescence-induced RNase cDNA clones, LX and LE, were isolated.
LX and LE gene expression is induced in leaves during advanced senescence, with LX showing higher induction.
LX gene expression is activated by ethylene in young leaves, while LE expression is induced by wounding and activated by abscisic acid.
Low expression in flowers and senescing leaves; no expression in stems, roots, or fruits.

Conclusions:

LX RNase likely functions in RNA catabolism during the final stages of leaf senescence.
LE RNase may function as a plant defense protein during wounding.
The study elucidates specific roles for LX and LE RNases in plant development and stress responses.

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