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Updated: Aug 9, 2026

Mouse Bladder Wall Injection
03:48

Mouse Bladder Wall Injection

Published on: July 12, 2011

Inhibition of basic fibroblast growth factor expression, angiogenesis, and growth of human bladder carcinoma in mice

C P Dinney1, D R Bielenberg, P Perrotte

  • 1Department of Cell Biology, The University of Texas M.D. Anderson Cancer Center, Houston 77030, USA.

Cancer Research
|March 4, 1998
PubMed

Insights

Interferon-alpha (IFN-alpha) inhibits basic fibroblast growth factor (bFGF) and angiogenesis, slowing human bladder cancer growth in mice. This suggests IFN-alpha has anti-angiogenic properties beyond its antiproliferative effects.

Area of Science:

  • Oncology
  • Immunology
  • Molecular Biology

Background:

  • Human transitional cell carcinoma exhibits high metastatic potential.
  • Basic fibroblast growth factor (bFGF) is implicated in tumor growth and angiogenesis.
  • Interferon-alpha (IFN-alpha) is known for its antiproliferative effects.

Purpose of the Study:

  • To investigate IFN-alpha's ability to inhibit bFGF expression in human bladder cancer.
  • To determine if IFN-alpha reduces tumor angiogenesis.
  • To assess the impact of IFN-alpha on bladder tumor growth in vivo.

Main Methods:

  • In vitro studies using 253J B-V and IFN-alpha-resistant 253J B-V IFNR cells.
  • Exposure to noncytostatic concentrations of IFN-alpha.
  • Implantation of cancer cells into the bladder wall of nude mice.
  • Systemic administration of IFN-alpha.

Main Results:

  • IFN-alpha down-regulated bFGF mRNA transcripts and protein production in vitro.
  • Systemic IFN-alpha decreased in vivo bFGF expression.
  • IFN-alpha reduced tumor blood vessel density (angiogenesis).
  • Tumor growth was inhibited in both IFN-alpha-insensitive and resistant cells.

Conclusions:

  • IFN-alpha inhibits human bladder cancer growth through anti-angiogenic mechanisms.
  • IFN-alpha reduces bFGF expression and angiogenesis in transitional cell carcinoma.
  • These findings add anti-angiogenic effects to the known antiproliferative actions of IFN-alpha.

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