Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Knowledge-based design of a soluble bacteriorhodopsin

C Gibas1, S Subramaniam

  • 1Center for Biophysics and Computational Biology, Beckman Institute for Advanced Science and Technology and National Center for Supercomputing Applications, University of Illinois, Urbana-Champaign, Urbana 61801, USA.

Protein Engineering
|March 6, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

AlignPCA-2D: PCA-reduced Euclidean vector alignment for 2D classification in cryo-EM.

Acta crystallographica. Section D, Structural biology·2026
Same author

Job insecurity and psychological wellbeing among junior doctors in Malaysia: A national cross-sectional study.

The Medical journal of Malaysia·2026
Same author

Cytological development, calcium dynamic and metabolite profile of stress-induced tobacco (Nicotiana tabacum L.) microspore under calcium chloride treatment.

Brazilian journal of biology = Revista brasleira de biologia·2025
Same author

Exploring the Utility of the Modified Hospitalized-Patient One-Year Mortality Risk Score to Trigger Referrals to Palliative Care for Inpatients With Cancer.

Cancer medicine·2024
Same author

Morpho-anatomical characterization and DNA barcoding of Artemesia vulgaris L.

Brazilian journal of biology = Revista brasleira de biologia·2024
Same author

Links between socio-demographic characteristics and body mass index to colorectal cancer in North Borneo, Malaysia: A case-control study.

The Medical journal of Malaysia·2023
Same journal

Structure of a human Rhinovirus complexed with its receptor molecule.

Protein engineering·2024
Same journal

pH-responsive polymer-assisted refolding of urea- and organic solvent-denatured alpha-chymotrypsin.

Protein engineering·2004
Same journal

Evaluation of different linker regions for multimerization and coupling chemistry for immobilization of a proteinaceous affinity ligand.

Protein engineering·2004
Same journal

Recombinant porcine intestinal carboxylesterase: cloning from the pig liver esterase gene by site-directed mutagenesis, functional expression and characterization.

Protein engineering·2004
Same journal

Periplasmic expression of human growth hormone via plasmid vectors containing the lambdaPL promoter: use of HPLC for product quantification.

Protein engineering·2004
Same journal

Shift of fibril-forming ability of the designed alpha-helical coiled-coil peptides into the physiological pH region.

Protein engineering·2004
See all related articles

Researchers designed a soluble, seven-helix bundle protein by applying rules from high-resolution protein structures. This approach aims to create proteins with desirable properties like easier crystallization, improving protein design strategies.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Protein Engineering

Background:

  • High-resolution protein structures provide valuable insights for rational protein design.
  • Soluble proteins often exhibit desirable properties like crystallization, unlike membrane proteins.

Purpose of the Study:

  • To apply knowledge from protein structures to redesign bacteriorhodopsin.
  • To create blueprints for a monomeric, soluble seven-helix bundle protein with improved properties.

Main Methods:

  • Extracted design rules from known protein structures.
  • Modified protein sequences, preserving essential functional residues.
  • Distributed polar and charged residues on the surface for solubility.
  • Increased apolar contacts in helix intercalation regions.

Related Experiment Videos

Main Results:

  • Developed a scheme for designing soluble seven-helix bundle proteins.
  • The design scheme requires knowledge of helix arrangement and contacts, not detailed structures.
  • Aimed to create a protein with an internal structure similar to native proteins and enhanced solubility.

Conclusions:

  • The study presents a method for designing novel soluble helical proteins.
  • This approach can yield proteins with desirable characteristics, such as improved crystallization potential.
  • The protein design strategy is applicable even without exhaustive structural data.