Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunoglobulins to group A streptococcal surface molecules decrease adherence to and invasion of human pharyngeal

U Fluckiger1, K F Jones, V A Fischetti

  • 1Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, New York, New York 10021, USA.

Infection and Immunity
|March 6, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bullous cellulitis with Cryptococcus neoformans.

Infection·2011
Same author

Survey of aspergillosis in non-neutropenic patients in Swiss teaching hospitals.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2010
Same author

[Lymphogranuloma venereum. An old disease in a new dress].

Der Internist·2010
Same author

[A drug addict presenting with complicated tricuspid valve endocarditis].

Der Internist·2010
Same author

European guidelines for antifungal management in leukemia and hematopoietic stem cell transplant recipients: summary of the ECIL 3--2009 update.

Bone marrow transplantation·2010
Same author

Prevalence of Bacillus anthracis-like organisms and bacteriophages in the intestinal tract of the earthworm Eisenia fetida.

Applied and environmental microbiology·2010
Same journal

<i>Porphyromonas gingivalis</i> sphingolipids affect early responses of THP-1 macrophages to outer membrane vesicles.

Infection and immunity·2026
Same journal

A genome-wide CRISPR screen defines host determinants of early <i>Brucella</i> infection in human macrophage-like cells.

Infection and immunity·2026
Same journal

Antiphospholipid antibodies in acute and post-treatment Lyme disease.

Infection and immunity·2026
Same journal

The cholesterol-dependent cytolysin promotes <i>Streptococcus</i> systemic spread and induces arachidonic acid accumulation-mediated lethality in a murine intraperitoneal infection model.

Infection and immunity·2026
Same journal

Phenotypic and genotypic analysis of <i>Candida albicans</i> vaginal isolates reveals that <i>ECE1</i> expression underpins pathogenicity.

Infection and immunity·2026
Same journal

<i>Staphylococcus aureus</i> pore-forming toxins differentially shape disease severity in experimental endophthalmitis.

Infection and immunity·2026
See all related articles

Secretory immunoglobulin A (sIgA) antibodies prevent Streptococcus pyogenes adherence to throat cells, while M protein-specific IgG antibodies inhibit bacterial invasion. These findings highlight the roles of antibodies in combating streptococcal infections.

Area of Science:

  • Microbiology
  • Immunology
  • Infectious Diseases

Background:

  • Group A Streptococcus (Streptococcus pyogenes) M protein is a key virulence factor in initial infection stages.
  • Acute pharyngitis, a common infection, is caused by Streptococcus pyogenes.
  • Understanding antibody roles in preventing streptococcal adherence and internalization to pharyngeal cells is crucial.

Purpose of the Study:

  • To investigate the inhibitory effects of secretory immunoglobulin A (sIgA) and immunoglobulin G (IgG) on Streptococcus pyogenes adherence and internalization to human pharyngeal cells.
  • To determine the specific roles of whole sIgA, M6 protein-specific sIgA, and M6 protein-specific serum IgG in these processes.

Main Methods:

  • Utilized Streptococcus pyogenes strains: D471 (M protein-positive) and JRS75 (M protein-negative).

Related Experiment Videos

  • Tested purified whole sIgA, M6 protein-specific sIgA, and M6 protein-specific IgG for their effects on streptococcal adherence and internalization to cultured human pharyngeal cells.
  • Employed sIgA preabsorbed with M protein to assess specificity.

    • Main Results:

    • Purified whole sIgA and M6 protein-specific sIgA significantly reduced streptococcal adherence to pharyngeal cells.
    • sIgA preabsorbed with M protein also decreased bacterial adherence, indicating M protein's role in this interaction.
    • M6 protein-specific IgG antibodies did not affect bacterial attachment but significantly reduced internalization.

    Conclusions:

    • Secretory IgA plays a critical role in preventing Streptococcus pyogenes infection at mucosal surfaces by inhibiting bacterial adherence.
    • M protein-specific IgG antibodies are important for blocking bacterial invasion by targeting specific epitopes involved in internalization.