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M-cell surface beta1 integrin expression and invasin-mediated targeting of Yersinia pseudotuberculosis to mouse
M A Clark1, B H Hirst, M A Jepson
1Department of Physiological Sciences, Medical School, University of Newcastle upon Tyne, United Kingdom. Ann.Clark@newcastle.ac.uk
Abstract:
Quantitative analysis of Yersinia pseudotuberculosis infection of murine gut loops revealed that significantly more wild-type bacteria associated with Peyer's patch M cells than with dome enterocytes or goblet cells. An invasin-deficient mutant was significantly attenuated for M-cell invasion, while beta1 integrin expression was demonstrated in the apical membranes of M cells but not enterocytes. M-cell targeting by Yersinia pseudotuberculosis in vivo may, therefore, be mediated primarily by the interaction of invasin with cell surface beta1 integrins.
Insights
Yersinia pseudotuberculosis preferentially targets M cells in the gut. This bacterial targeting is primarily mediated by invasin interacting with beta1 integrins on M cells.
Area of Science:
- Microbiology
- Immunology
- Gastroenterology
Background:
- Yersinia pseudotuberculosis is a pathogen that infects the mammalian gut.
- Understanding pathogen-host interactions is crucial for controlling infections.
Purpose of the Study:
- To investigate the specific cell types targeted by Yersinia pseudotuberculosis within the murine gut.
- To elucidate the molecular mechanisms underlying M-cell targeting by Yersinia pseudotuberculosis.
Main Methods:
- Quantitative analysis of bacterial association with different gut cell types in murine gut loops.
- Assessment of M-cell invasion by wild-type and invasin-deficient Yersinia pseudotuberculosis mutants.
- Immunohistochemical detection of beta1 integrin expression in M cells and enterocytes.
Main Results:
- Wild-type Yersinia pseudotuberculosis showed significantly higher association with Peyer's patch M cells compared to dome enterocytes and goblet cells.
- An invasin-deficient mutant exhibited significantly reduced M-cell invasion.
- Beta1 integrin was expressed on the apical membranes of M cells but not enterocytes.
Conclusions:
- Yersinia pseudotuberculosis preferentially associates with M cells in the murine gut.
- Invasin-mediated interaction with beta1 integrins on M cells is a key mechanism for Yersinia pseudotuberculosis targeting in vivo.