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Localization of the zona glycoproteins ZPB (ZP3 alpha) and ZPC (ZP3 beta) in the bovine ovary during pre- and
I Totzauer1, S Kölle, F Sinowatz
1Lehrstuhl für Tieranatomie II, LMU München, Germany.
Abstract:
The purpose of this study was to investigate the expression of two zona pellucida gene families ZPB (ZP3 alpha) and ZPC (ZP3 beta). Sections of ovaries from bovine fetuses, calves and cows were labelled with polyclonal antibodies. Immunopositive labelling was found in both the follicle cells and the oocyte. Labelling was dependent on the stage of development. The specific sequence of immunopositive reactions suggests that in the bovine fetus both the ovary and the follicle cells contribute to the production of the zona pellucida during pre- and postnatal development.