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Alteration in myosatellite cell commitment with muscle maturation

J Yang1, R Kelly, M Daood

  • 1Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pennsylvania 15261, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|March 7, 1998
PubMed
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Myosatellite cells in mice show reduced commitment to fast myosin light chain 3F (MLC3F) gene expression as muscles mature. This suggests developmental changes influence muscle fiber type programming in these key cells.

Area of Science:

  • Muscle biology
  • Cellular and molecular biology
  • Developmental biology

Background:

  • Myosatellite cells are crucial for muscle regeneration and growth.
  • These cells are precursors to myoblasts and differentiate into muscle fibers.
  • Understanding their programming is key to muscle development and disease.

Purpose of the Study:

  • To investigate the developmental commitment of myosatellite cells to express specific contractile protein isoforms.
  • To evaluate how muscle maturation affects the expression of fast myosin light chain 3F (MLC3F) in myosatellite cells.
  • To determine if myosatellite cells retain their developmental program into adulthood.

Main Methods:

  • Used transgenic mice expressing a reporter gene (nlsbeta-gal) under the control of the MLC3F gene.

Related Experiment Videos

  • Isolated and cultured myosatellite cells from neonatal and adult soleus and EDL muscles.
  • Assessed transgene expression in differentiated myocytes and myotubes to quantify MLC3F gene commitment.

    • Main Results:

    • Transgene expression, indicating MLC3F commitment, was significantly higher in neonatal myosatellite cells (90% in EDL, 70% in soleus) compared to adult cells (61% in adult EDL, 32% in adult soleus).
    • Myosatellite cells from adult muscles showed a reduced capacity to express the fast MLC3F transgene compared to their neonatal counterparts.
    • This age-related decrease suggests an alteration in myosatellite cell commitment during muscle maturation.

    Conclusions:

    • Myosatellite cell commitment to express fast MLC3F decreases with muscle maturation.
    • This finding supports the hypothesis that MLC3F is downregulated in myofibers transitioning to a slower phenotype.
    • Developmental programming influences the fiber-type specificity of differentiated myosatellite cells.