Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tissue factor regulates plasminogen binding and activation

Z Fan1, P J Larson, J Bognacki

  • 1Department of Pathology, University of Pennsylvania, Philadelphia, PA 19104, USA.

Blood
|April 16, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Section-level genome sequencing and comparative genomics of <i>Aspergillus</i> sections <i>Cavernicolus</i> and <i>Usti</i>.

Studies in mycology·2025
Same author

Significant hindlimb static weight-bearing asymmetry persists for 40-weeks in a longitudinal study in two widely used rat models of surgically induced osteoarthritis knee pain.

Frontiers in pharmacology·2025
Same author

J-2156, a small molecule somatostatin type 4 receptor agonist, alleviated hindpaw hypersensitivity in the streptozotocin-induced rat model of painful diabetic neuropathy but with a 2-fold decrease in potency at an advanced stage in the model, mimicking morphine.

Frontiers in pharmacology·2024
Same author

Maternal and Child Health Pipeline Training Programs: A Description of Training Across 6 Funded Programs.

Maternal and child health journal·2022
Same author

Serial femtosecond and serial synchrotron crystallography can yield data of equivalent quality: A systematic comparison.

Science advances·2021
Same author

101 <i>Dothideomycetes</i> genomes: A test case for predicting lifestyles and emergence of pathogens.

Studies in mycology·2020
Same journal

Fibrocytes drive JAK2V617F-mutated myelofibrosis: pitavastatin reverses marrow fibrosis and anemia.

Blood·2026
Same journal

Identifying steroid-refractory aGVHD before it happens.

Blood·2026
Same journal

ELISA-negative HIT: antibody recognition and relevance.

Blood·2026
Same journal

EBV and immunodeficiency: the odd couple drawn to the brain.

Blood·2026
Same journal

A bone to pick with ferric carboxymaltose.

Blood·2026
Same journal

A step toward streamlining HIT diagnosis.

Blood·2026
See all related articles

Tissue factor (TF) binds plasminogen, enhancing its activation and inhibiting fibrinolysis. This interaction, mediated by TF

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Tissue factor (TF) is crucial in hemostasis and thrombosis.
  • TF and plasminogen activators share roles in processes like atherogenesis and angiogenesis.
  • Interactions between TF and the plasminogen activator system are not fully understood.

Purpose of the Study:

  • To investigate the interaction between tissue factor (TF) and the plasminogen activator system.
  • To determine if plasminogen binds to TF and if TF modulates plasminogen activation.

Main Methods:

  • Optical biosensor interaction analysis to detect plasminogen binding to TF apoprotein.
  • Cell-based assays using Chinese hamster ovary (CHO) cells expressing TF.
  • Examination of plasmin formation via SDS-PAGE.

Related Experiment Videos

  • Inhibition assays for fibrinolysis and plasminogen binding.

    • Main Results:

    • Plasminogen directly binds to the extracellular domain of TF apoprotein via its kringle 1-3 domains.
    • TF expression on cells significantly increases plasminogen binding.
    • TF enhances urokinase-type plasminogen activator (uPA)-mediated plasminogen activation.
    • TF inhibits fibrinolysis and plasminogen binding to endothelial cells and trophoblasts.
    • TF and plasminogen colocalize in atherosclerotic plaques.

    Conclusions:

    • A high-affinity interaction exists between TF and plasminogen, mediated by TF's extracellular domain and plasminogen's kringle 1-3 regions.
    • TF modulates plasminogen localization and activation at extravascular sites.
    • This interaction has implications for TF's role in atherogenesis and other TF-mediated processes.