Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Directed termination PCR: a one-step approach to mutation detection

J Chen1, P D Hebert

  • 1Department of Zoology, University of Guelph, Guelph, Ontario N1G 2W1, Canada. jjchen@uoguelph.ca

Nucleic Acids Research
|April 29, 1998
PubMed
Summary

A new polymerase chain reaction (PCR) method generates nested termination fragments in one step. This technique efficiently detects DNA sequence variations, including insertions, deletions, and substitutions, up to 1 kb.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Regulation and immunolocalization of acyl-coenzyme A: cholesterol acyltransferase in mammalian cells as studied with specific antibodies.

The Journal of biological chemistry·1995
Same author

Regulation of platelet activation in vitro by the c-Mpl ligand, thrombopoietin.

Blood·1995
Same author

Operator-less processing of myocardial perfusion SPECT studies.

Journal of nuclear medicine : official publication, Society of Nuclear Medicine·1995
Same author

Regulation of delta FosB and FosB-like proteins by electroconvulsive seizure and cocaine treatments.

Molecular pharmacology·1995
Same author

T cells, but not B cells, are required for bowel inflammation in interleukin 2-deficient mice.

The Journal of experimental medicine·1995
Same author

Regulation of cytochrome P450 2C11 (CYP2C11) gene expression by interleukin-1, sphingomyelin hydrolysis, and ceramides in rat hepatocytes.

The Journal of biological chemistry·1995

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Detecting DNA sequence variations is crucial for genetic research and diagnostics.
  • Existing methods for sequence variation detection can be complex and time-consuming.

Purpose of the Study:

  • To develop a novel, single-step method for generating nested termination fragments.
  • To enable efficient detection of insertions/deletions and single base pair substitutions.

Main Methods:

  • A novel polymerase chain reaction (PCR)-based method was developed.
  • The method integrates selective DNA amplification and directed chain termination into a single PCR reaction.
  • Generated termination fragments are analyzed on polyacrylamide gels.

Main Results:

Related Experiment Videos

  • The described PCR method successfully generates nested termination fragments.
  • The technique allows for the detection of both insertions/deletions and single base pair substitutions.
  • The method is effective for analyzing DNA sequences up to 1 kb in length.

Conclusions:

  • This one-step PCR-based approach offers a highly effective strategy for sequence variation detection.
  • The method simplifies the process of identifying genetic alterations.
  • The technique has broad applicability in genetic analysis and research.