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Ultra-wide band electromagnetic radiation does not affect UV-induced recombination and mutagenesis in yeast
O N Pakhomova1, M L Belt, S P Mathur
1U.S. Army Medical Research Detachment, Walter Reed Army Institute of Research, Brooks Air Force Base, San Antonio, Texas 78235-5324, USA. pakhomov@netxpress.com
Abstract:
Cell samples of the yeast Saccharomyces cerevisiae were exposed to 100 J/m2 of 254 nm ultraviolet (UV) radiation followed by a 30 min treatment with ultra-wide band (UWB) electromagnetic pulses. The UWB pulses (101-104 kV/m, 1.0 ns width, 165 ps rise time) were applied at the repetition rates of 0 Hz (sham), 16 Hz, or 600 Hz. The effect of exposures was evaluated from the colony-forming ability of the cells on complete and selective media and the number of aberrant colonies. The experiments established no effect of UWB exposure on the UV-induced reciprocal and non-reciprocal recombination, mutagenesis, or cell survival.