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Effect of inline filtration on ViaSpan cold-storage solution
B J Mueller1, S A Guessford, T T Chen
1DuPont Merck Pharmaceutical Company, Wilmington, DE 19880, USA.
Summary
Inline filtration effectively removes particulates from ViaSpan cold-storage solution, enhancing its safety for organ preservation. This process ensures the solution
Area of Science:
- Organ preservation solutions
- Medical device filtration technology
Background:
- ViaSpan is a widely used cold-storage solution for organ transplantation.
- Particulate contamination in preservation solutions can pose risks during clinical application.
Purpose of the Study:
- To evaluate the efficacy of inline filtration in reducing particulate load in ViaSpan.
- To assess the compatibility of ViaSpan and its common additives with inline blood transfusion filters.
Main Methods:
- Utilized commercially available inline blood transfusion filters (SQ40S, Pall Biomedical).
- Employed a light-obscuration technique to count particles before and after filtration.
- Analyzed the concentrations of ViaSpan constituents and drug additives post-filtration.
Main Results:
- Filtration removed over 99% of particles > 25 microm, 71% of particles > 10-25 microm, and 56% of particles 5-10 microm.
- Filtration did not significantly alter the concentrations of ViaSpan ingredients or common drug additives.
- Adequate flow rates were maintained with the inline filters.
Conclusions:
- Inline filtration is an effective method for reducing particulate contamination in ViaSpan.
- The filtration process does not compromise the integrity or efficacy of ViaSpan or its additives.
- This technique offers a safe and practical approach to enhance the quality of organ preservation solutions.
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