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[A mobile genetic element on a Bordetella pertussis chromosome stimulates cointegrate formation in Escherichia coli
M Iu Kirillov1, E V Nechaeva, G I Karataev
1Gamaleya Research Institute of Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow, Russia.
Abstract:
This work continues a series of reports devoted to the study of a repeated sequence isolated from the Bordetella pertussis chromosome (RSBP--Repeated Sequence of Bordetella Pertussis). The RSBP was earlier demonstrated to have a structure similar to IS elements and exhibit some properties of mobile genetic elements. The results of this work demonstrate the ability of this novel genetic element to stimulate the formation of cointegrates between independent replicons. We have studied the structure of five cointegrates formed by integration of a resident RSBP-containing plasmid into three different sites of a recipient replicon. The studied cointegrates are capable of resolution with the release of a resident plasmid independent of the host cell RecA function.