Study of semen stains by scanning electron microscopy. Influence of their ageing

In order to evaluate the degree of morphological preservation of human spermatozoa in semen stains, an experimental stain on cotton tissue was made and stored at room temperature for 12 months. Small pieces were cut at different times along this period, fixed in saline formaldehyde solution and examined by scanning electron microscopy. Results revealed that the preservation of spermatozoa integrity is high, which represents an important advantage for its use in Court.