Cloning of the human NCNF gene
Related Experiment Video
Updated: Oct 2, 2026

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells
Published on: July 29, 2015
S Schneider-Hirsch1, U M Bauer, R Heiermann
1Institute of Physiological Chemistry and Pathobiochemistry, Johannes Gutenberg-University Medical School, Mainz, Germany.
Abstract:
We have cloned from a cDNA library of human testis tissue the human homologue to the mouse nuclear orphan receptor NCNF (neuronal cell nuclear factor). The open reading frame encodes a protein of 480 amino acids, the sequence of which (EMBL accession no. X99975) is 98.3% identical to the mouse homologue. Northern blot analysis of adult human tissues revealed a broad pattern of tissue expression. Similar to NCNF expression in mouse testis, two transcript forms of the single copy gene are expressed in human tissues. The two transcript forms which differ only in their 3'UTR, result in human from differential polyadenylation, in mouse from alternative splicing. Based on the high level of sequence identity of human and murine NCNF, it is likely that also the human nuclear receptor is involved in the control of neurogenesis and gametogenesis.
Related Concept Videos
Reproductive Cloning
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Introduction to Nuclear Reprogramming
Cloning of Dolly the Sheep
Methods of Nuclear Reprogramming

