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Related Experiment Videos

Apatite induction by insoluble dentin collagen

T Saito1, M Yamauchi, M A Crenshaw

  • 1Dental Research Center, University of North Carolina at Chapel Hill, 27599-7455, USA.

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
|March 12, 1998
PubMed
Summary

Bound phosphate on dentin collagen is essential for initiating apatite formation. Removing phosphate significantly delays or prevents mineral induction, highlighting its critical role in biomineralization.

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Area of Science:

  • Biomineralization
  • Biochemistry
  • Materials Science

Background:

  • Phosphoproteins are implicated in dentin and bone mineral formation.
  • A portion of these phosphoproteins is bound to collagen matrices.
  • The precise role of bound phosphate in initiating mineralization is not fully understood.

Purpose of the Study:

  • To investigate the necessity of bound phosphate for mineral induction by isolated dentin collagen.
  • To quantify the effect of dephosphorylation on the mineral induction capacity of dentin collagen.
  • To determine the energetics of heterogeneous nucleation on phosphorylated collagen.

Main Methods:

  • Isolated bovine dentin collagen was demineralized using ethylenediaminetetraacetic acid (EDTA).
  • Collagen was incubated in supersaturated solutions to assess apatite induction.
Keywords:
Non-programmatic

Related Experiment Videos

  • Enzymatic dephosphorylation was used to progressively remove phosphate groups.
  • Interfacial tension for mineral induction was calculated based on experimental data.

    • Main Results:

    • Dentin collagen with intact phosphate groups induced apatite formation.
    • Progressive dephosphorylation increased mineral induction times, with no induction occurring after 90% phosphate removal.
    • Bone collagen with lower phosphate content did not induce mineralization.
    • Calculated interfacial tension for induction was 90 ergs/cm², similar to phosphorylated surfaces.

    Conclusions:

    • Bound phosphate on dentin collagen is critical for initiating apatite (mineral) formation.
    • Immobilized phosphoprotein on collagen facilitates ordered mineral growth.
    • The energetics of nucleation on phosphorylated surfaces are key to biomineralization processes.