Abnormalities in expression of genes, mRNA, and proteins of transforming growth factor-beta receptor type I and type

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Updated: Oct 30, 2026

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
12:34

Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome

Published on: April 2, 2018

K Fujiwara1, H Ikeda, T Yoshimoto

  • 1Department of Neurosurgery, Tohoku University School of Medicine, Sendai, Japan.

Clinical Neuropathology
|March 13, 1998
PubMed

Insights

Investigating transforming growth factor-beta (TGF-beta) receptors in pituitary tumors revealed altered messenger ribonucleic acid (mRNA) and protein expression. These changes, linked to transcriptional abnormalities, may impact tumor growth and bioactivity.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Oncology

Background:

  • Pituitary adenomas are common tumors with complex growth regulation.
  • Transforming growth factor-beta (TGF-beta) signaling, mediated by T beta R-I and T beta R-II, plays a role in cell growth and differentiation.
  • Abnormalities in TGF-beta receptor expression may contribute to pituitary tumor development and behavior.

Purpose of the Study:

  • To investigate abnormalities in deoxyribonucleic acid (DNA), messenger ribonucleic acid (mRNA), and protein expression of TGF-beta receptor type I (T beta R-I) and TGF-beta receptor type II (T beta R-II) in human pituitary adenomas.
  • To determine the correlation between mRNA and protein expression levels of these receptors.
  • To explore the potential implications of altered receptor expression on pituitary tumor bioactivity.

Main Methods:

  • Analysis of 33 human pituitary adenomas.
  • Reverse transcription-polymerase chain reaction (RT-PCR) for mRNA extraction and complementary DNA synthesis.
  • Polymerase chain reaction (PCR) and single-strand conformation polymorphism (SSCP) for DNA abnormality screening.
  • Direct sequencing of suspected abnormal DNA regions.
  • Quantitative PCR for mRNA expression measurement.
  • Immunohistochemical staining for protein expression quantification.

Main Results:

  • No gene mutations were detected in the screened regions.
  • A single base substitution (polymorphism) in the T beta R-II gene (codon 389) was identified in 10 out of 28 cases.
  • Significant correlation observed between mRNA and protein expression levels of T beta R-I and T beta R-II.
  • Variations in T beta R-I and T beta R-II protein expression were noted among different tumors.

Conclusions:

  • Differences in T beta R-I and/or T beta R-II mRNA expression in pituitary tumors are likely due to transcriptional abnormalities.
  • Altered expression of TGF-beta receptors may influence TGF-beta-mediated growth control in pituitary tumors.
  • These expression changes may serve as indicators of pituitary tumor bioactivity.

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