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Related Experiment Videos

Metabolic engineering of propanediol pathways

D C Cameron1, N E Altaras, M L Hoffman

  • 1Department of Chemical Engineering, University of Wisconsin-Madison 53706-1691, USA. cameron@engr.wisc.edu

Biotechnology Progress
|March 13, 1998
PubMed
Summary

Metabolic engineering enables new microbial fermentation processes for producing 1,3-propanediol (1,3-PD) from sugars and 1,2-propanediol (1,2-PD) from glucose. These advancements promise high yields and straightforward purification for bio-based chemical production.

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Area of Science:

  • Biotechnology
  • Metabolic Engineering
  • Industrial Microbiology

Background:

  • Microbial fermentation is key for converting renewable resources into valuable chemicals.
  • Developing efficient fermentation routes for 1,3-propanediol (1,3-PD) from sugars, unlike glycerol, is a significant challenge.
  • Identifying and engineering organisms for 1,2-propanediol (1,2-PD) production from various sugars is crucial.

Purpose of the Study:

  • To develop novel fermentation processes for producing 1,3-propanediol (1,3-PD) and 1,2-propanediol (1,2-PD) using metabolic engineering.
  • To explore and optimize microbial conversion of sugars and glycerol into 1,3-PD.
  • To engineer strains for efficient sugar-based 1,2-PD production and analyze the potential of fermentation for propanediol synthesis.

Main Methods:

Related Experiment Videos

  • Fed-batch fermentation of glycerol to 1,3-PD using Klebsiella pneumoniae.
  • Investigating mixed-culture fermentation, cofermentation, and metabolic engineering for sugar-to-1,3-PD conversion.
  • Engineering Escherichia coli strains for glucose to (R)-1,2-PD conversion via aldose reductase or glycerol dehydrogenase.
  • Fermentation of sugars to 1,2-PD by Thermoanaerobacterium thermosaccharolyticum in batch and continuous cultures.
  • Linear optimization studies to assess theoretical fermentation yields and product titers.

Main Results:

  • Klebsiella pneumoniae efficiently ferments glycerol to 1,3-PD.
  • Initial gene expression studies for a sugar-to-1,3-PD pathway in Saccharomyces cerevisiae were reported.
  • Thermoanaerobacterium thermosaccharolyticum demonstrated effective fermentation of various sugars to 1,2-PD.
  • Engineered E. coli produced (R)-1,2-PD from glucose.
  • Optimization studies suggest high propanediol yields (approaching theoretical maximum) and titers (up to 100 g/L) are achievable with CO2 as the primary coproduct under aerobic conditions.

Conclusions:

  • Metabolic engineering is vital for developing advanced fermentation processes.
  • Efficient production of 1,3-PD and 1,2-PD from renewable resources is feasible through engineered microbial systems.
  • High product titers and simplified purification processes are attainable, enhancing the economic viability of bio-based propanediol production.