Anti-inflammatory activity in mice of extracts from Mediterranean marine invertebrates

The effects of dichloromethane and methanol extracts from the marine invertebrates Leptogorgia ceratophyta, Holothuria tubulosa, Coscinasterias tenuispina and Phallusia fumigata on carrageenan-induced paw oedema in mice were investigated. The dichloromethane extract of Coscinasterias tenuispina and the methanol extract of Holothuria tubulosa administered p.o. at 50, 100 and 150 mg/kg, inhibited oedema in a dose-dependent manner 3 h after administration of carrageenan. Both extracts partially decreased elastase activity and PGE2 levels measured in homogenates from inflamed paws, without affecting the levels of this prostanoid present in stomach homogenates. As observed with the selective inhibitor NS398, both extracts can decrease cyclo-oxygenase activity in inflamed tissues but do not modify the constitutive cyclo-oxygenase enzyme. Therefore, these extracts represent new marine resources for the isolation of novel agents active on inflammatory conditions.