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Updated: Jul 20, 2026

A Quantitative Cell Migration Assay for Murine Enteric Neural Progenitors
Published on: September 18, 2013
Epidermal growth factor receptor-stimulated intestinal epithelial cell migration requires phospholipase C activity
1Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-2576, USA. d-brent.polk@mcmail.vanderbilt.edu
Background & Aims:
Ulceration of intestinal mucosa is rapidly followed by enterocyte migration via restitution. The aim of this study was to investigate signaling mechanisms of epidermal growth factor (EGF) receptor-stimulated monolayer restitution in a mouse intestinal epithelial cell line.
Methods:
EGF-stimulated cell migration was determined using a wound model in the presence of agonists and/or antagonists of tyrosine kinase, phospholipase C, phosphatidylinositol 3-kinase, or protein kinase C. The tyrosine phosphorylation state of the EGF receptor, phosphatidylinositol phospholipase C gamma1 (PLCgamma1), focal adhesion kinase, and cellular lysates was determined by immunodetection.
Results:
EGF stimulated cell migration twofold at 4, 8, and 24 hours. Inhibition of EGF receptor tyrosine kinase activity, phospholipase C, or phosphatidylinositol 3-kinase attenuated EGF-induced intestinal cell migration. Pretreatment of cells with phorbol 12-myristate 13-acetate, known to down-regulate protein kinase C expression, blocked EGF-induced cell migration. Increased tyrosine phosphorylation of the EGF receptor and PLCgamma1 was detected within 5 minutes after wounding.
Conclusions:
EGF-stimulated intestinal cell migration requires intact EGF receptor tyrosine kinase, phospholipase, and protein kinase C activities. PLCgamma1 may be a key regulatory molecule in the initial EGF receptor signal transduction pathway of EGF-stimulated cell migration.
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