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Age estimation from racemization rate using heated teeth

S Ohtani1, Y Yamada, I Yamamoto

  • 1Department of Forensic Dental Medicine, Kanagawa Dental College, Yokosuka, Japan.

The Journal of Forensic Odonto-Stomatology
|June 1, 1997
PubMed
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The racemization ratio (D/L ratio) of aspartic acid in dentine can estimate age, especially in burned bodies. Soluble peptide D/L ratios accurately predict age in heated teeth, unlike total amino acids.

Area of Science:

  • Forensic science
  • Biogeochemistry
  • Paleodontology

Background:

  • Amino acid racemization is a dating method.
  • Dentine composition affects racemization.
  • Heat exposure alters amino acid D/L ratios.

Purpose of the Study:

  • To assess aspartic acid racemization in dentine.
  • To evaluate age estimation accuracy in heated vs. unheated teeth.
  • To determine the reliability of soluble peptide vs. total amino acid D/L ratios for age estimation in burned human remains.

Main Methods:

  • Extraction of soluble peptide and total amino acid from dentine.
  • Measurement of aspartic acid racemization (D/L ratio).
  • Comparison of estimated ages with true ages for heated and unheated teeth.

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Main Results:

  • Total amino acid D/L ratios were higher in heated teeth.
  • Soluble peptide D/L ratios remained consistent between heated and unheated teeth.
  • Age estimation from soluble peptide D/L ratios was accurate for burned teeth.

Conclusions:

  • Dentine soluble peptide D/L ratio is a reliable indicator for age estimation in burned individuals.
  • Total amino acid D/L ratio is less reliable for age estimation in heated teeth due to accelerated racemization.
  • This method offers a valuable tool for forensic age assessment in challenging cases.