Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High resolution single strand conformation polymorphism analysis using formamide and ethidium bromide staining

T Xie1, S L Ho, O C Ma

  • 1University Department of Medicine, University of Hong Kong, Hong Kong.

Molecular Pathology : MP
|March 14, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Cognitive function, brain MRI characteristics and their association with blood phenylalanine levels in patients with phenylketonuria].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2026
Same author

[Gastrointestinal defects and immunodeficiency syndrome related to TTC7A gene variants in 3 children].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2026
Same author

Corrigendum to "AdipoRon ameliorates anxiety- and depression-like behaviors in chronic restraint-stressed mice via AMPK-PPARα-BDNF-TrkB signaling" [Eur. J. Pharmacol. (2026) 1011 178468 doi: 10.1016/j.ejphar.2025.178468].

European journal of pharmacology·2025
Same author

Tislelizumab plus nab-paclitaxel and cisplatin as induction immunochemotherapy for organ preservation of locally advanced hypopharyngeal squamous cell carcinoma: a single-arm phase II trial.

ESMO open·2025
Same author

Perineural Invasion Exhibits Traits of Neurodegeneration.

Journal of dental research·2025
Same author

[Rituximab combined with intensive immunochemotherapy for sporadic adult Burkitt lymphoma: efficacy and prognosis analyse].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2025
Same journal

Development of molecular methods for the identification of aspergillus and emerging moulds in paraffin wax embedded tissue sections.

Molecular pathology : MP·2003
Same journal

Tempero-spatial dissociation between the expression of Fas and apoptosis after coronary occlusion.

Molecular pathology : MP·2003
Same journal

Inverse correlation between high level expression of cyclin E and proliferation index in transitional cell carcinoma of the bladder.

Molecular pathology : MP·2003
Same journal

Nuclear beta catenin as a potential prognostic and diagnostic marker in patients with colorectal cancer from Hong Kong.

Molecular pathology : MP·2003
Same journal

Increased cyclin D1 expression in cancer of the ampulla of Vater: relevance to nuclear beta catenin accumulation and k-ras gene mutation.

Molecular pathology : MP·2003
Same journal

Expression of the cell cycle regulatory proteins p34cdc2, p21waf1, and p53 in node negative invasive ductal breast carcinoma.

Molecular pathology : MP·2003
See all related articles

Formamide enhances single-strand conformation polymorphism (SSCP) analysis for improved DNA fragment analysis. This method offers high sensitivity and resolution for detecting structural DNA variations.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Single-strand conformation polymorphism (SSCP) is a widely used method for detecting DNA sequence variations.
  • Traditional SSCP methods can be limited in sensitivity and resolution, particularly for longer DNA fragments.

Purpose of the Study:

  • To improve the resolution and sensitivity of SSCP analysis.
  • To evaluate the efficacy of formamide as a denaturant in SSCP.

Main Methods:

  • Modified SSCP analysis incorporating formamide as a denaturant.
  • Testing the method on 14 PCR samples from two different genes, including a fragment near 250 base pairs.

Main Results:

  • The addition of formamide significantly increased the resolution of SSCP.

Related Experiment Videos

  • The modified method achieved 100% sensitivity in discriminating between DNA samples.
  • Effective detection of structural differences in DNA fragments, even long ones.

    • Conclusions:

    • Formamide is a valuable additive for enhancing SSCP analysis.
    • This modified procedure offers a rapid, simple, safe, and highly sensitive approach for DNA fragment analysis.
    • The enhanced SSCP method is suitable for detecting structural DNA variations.