Method for the preparation of restricted access media by low-temperature plasma treatment

A new method for the preparation of restricted access media (RAM) was developed. A low-temperature plasma treatment removed octadecyl groups on the external surface of octadecylsilylated silica gel before those on the internal surface to produce silanol groups. The silanol groups produced were glycerylpropylsilylated to give RAM, DIOL-ODSs, consisting of internal octadecyl groups and external glycerylpropyl groups. DIOL-ODSs were inert to serum proteins and retained low-molecular-mass analytes adequately. Direct injection analysis of anticonvulsants in serum was accurately performed by column-switching high-performance liquid chromatography using a pre-column packed with DIOL-ODS.