Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chimeric measles viruses with a foreign envelope

P Spielhofer1, T Bächi, T Fehr

  • 1Institute of Molecular Biology Division I, University of Zürich, Switzerland.

Journal of Virology
|March 14, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Role of herpes simplex virus type 1 glycoprotein B in virus assembly].

Virologie (Montrouge, France)·2021
Same author

Biochemical basis of Fabry disease with emphasis on mitochondrial function and protein trafficking.

Advances in clinical chemistry·2009
Same author

Reverse genetics of measles virus and resulting multivalent recombinant vaccines: applications of recombinant measles viruses.

Current topics in microbiology and immunology·2009
Same author

Demonstration of lipids in plastic resin-embedded sections of skin material.

Journal of microscopy·2009
Same author

Insights into post-translational processing of beta-galactosidase in an animal model resembling late infantile human G-gangliosidosis.

Journal of cellular and molecular medicine·2007
Same author

Interaction between the P1 protein of Mycoplasma pneumoniae and receptors on HEp-2 cells.

Microbiology (Reading, England)·2007

Researchers created novel measles virus (MV) chimeras with vesicular stomatitis virus (VSV) envelopes. These chimeric viruses show potential for developing new vaccines and gene therapies.

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Measles virus (MV) and vesicular stomatitis virus (VSV) belong to the Mononegavirales order but are distantly related.
  • Understanding viral assembly and host range determination is crucial for vaccine development.

Purpose of the Study:

  • To generate and characterize genetically stable chimeric viruses between MV and VSV.
  • To investigate the role of viral matrix (M) protein and envelope glycoproteins in virus assembly and host range.
  • To evaluate the potential of these chimeras as vaccine candidates.

Main Methods:

  • Construction of two chimeric viruses: MGV (encoding VSV G) and MG/FV (encoding a VSV G/MV F hybrid).
  • Analysis of viral protein content, particularly the matrix (M) protein, in chimeric virions.

Related Experiment Videos

  • Assessment of viral transcription, replication, and assembly processes.
  • Immunization of mice with chimeric viruses and challenge with wild-type VSV.

    • Main Results:

    • MGV virions assembled without the MV matrix (M) protein, demonstrating M-independent assembly.
    • The cytoplasmic domain of MV F protein facilitated M incorporation and enhanced assembly in MG/FV.
    • Chimeric virus formation was delayed, with titers reduced approximately 50-fold compared to standard MV.
    • Host range was dictated by envelope glycoproteins, while transcription and replication were MV-mediated.
    • Mice immunized with chimeras were protected against lethal wild-type VSV challenge.

    Conclusions:

    • Virus assembly can occur independently of the matrix (M) protein.
    • Envelope glycoproteins play a critical role in determining viral host range.
    • These chimeric viruses represent a promising platform for developing novel vaccines and gene therapeutics.