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Updated: Aug 4, 2026
12:19
Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
Cell cycle control of chorion gene amplification
B R Calvi1, M A Lilly, A C Spradling
1Howard Hughes Medical Institute Research Laboratories, Carnegie Institution of Washington, Baltimore, Maryland 21210, USA.
Genes & Development
|April 16, 1998
Summary
Drosophila chorion gene amplification occurs in two phases, regulated by Cyclin E. This process is crucial for eggshell production and provides a model for DNA replication control.
Area of Science:
- Cell Biology
- Developmental Biology
- Genetics
Background:
- Over-replication of chorion genes in Drosophila is vital for rapid eggshell biosynthesis.
- The precise regulation of this gene amplification in relation to cell cycles was previously unclear.
Purpose of the Study:
- To investigate the regulation of chorion gene amplification during oogenesis.
- To understand the relationship between gene amplification and follicle cell cycles.
Main Methods:
- Developed a technique combining BrdU incorporation and FISH to detect amplifying chorion genes in individual follicle cells.
- Analyzed Cyclin E expression patterns during oogenesis.
Main Results:
- Chorion gene amplification occurs in two distinct developmental phases: periodic amplification during S phases of endocycles and continuous amplification after endocycles cease.
- Late-phase amplification initiates synchronously in follicle cells.
- Cyclin E expression patterns correlate with the two amplification phases, and Cyclin E is required for amplification.
Conclusions:
- Cyclin E plays a dual role, positively regulating amplification and negatively inhibiting re-replication within a cell cycle.
- Specific chorion gene origins may possess factors allowing escape from negative re-replication control.
- Chorion gene amplification serves as a model for metazoan replicons and cell cycle-restricted replication.
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