Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative video microscopy

D E Wolf1

  • 1Department of Physiology, University of Massachusetts Medical School, Worcester 01655, USA.

Methods in Cell Biology
|March 21, 1998
PubMed
Summary
This summary is machine-generated.

This guide offers practical solutions for accurate image quantitation in video microscopy. It addresses common pitfalls like aspect ratio errors and improper dynamic range settings to ensure reliable results.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Anisotropy of electromigration-induced void and island drift.

Journal of physics. Condensed matter : an Institute of Physics journal·2013
Same author

From nanoparticles to nanocrystalline bulk: percolation effects in field assisted sintering of silicon nanoparticles.

Nanotechnology·2011
Same author

Biotin; synthesis of dl-biotin, dl-allobiotin and dl-epi-allobiotin.

Journal of the American Chemical Society·2010
Same author

Biotin; resolution of dl-biotin.

Journal of the American Chemical Society·2010
Same author

The structure of rhizopterin.

Journal of the American Chemical Society·2010
Same author

Failure and success of hydrodynamic interaction models.

The European physical journal. E, Soft matter·2008
Same journal

Quantification of cell viability by automated analysis of live cell imaging.

Methods in cell biology·2026
Same journal

Flow cytometry evaluation of cytotoxicity exerted by effector immune cells against tumor cells.

Methods in cell biology·2026
Same journal

Time-lapse confocal laser scanning microscopy analysis of FOOD formation.

Methods in cell biology·2026
Same journal

Screening and identification of protein-protein interaction using proximity labeling.

Methods in cell biology·2026
Same journal

Quantitative high-content profiling of mitochondrial morphology with automated statistical analysis and integrated data visualization.

Methods in cell biology·2026
Same journal

Super-resolution imaging of cell death in Drosophila tissues via expansion and pan-expansion microscopy.

Methods in cell biology·2026
See all related articles

Area of Science:

  • Microscopy
  • Image Analysis
  • Scientific Imaging

Background:

  • Accurate image quantitation is crucial in scientific research.
  • Variations in video microscopy systems can lead to measurement errors.
  • Understanding common technical issues is essential for reliable data.

Purpose of the Study:

  • To provide a practical guide for accurate image quantitation.
  • To highlight critical issues that compromise image analysis success.
  • To offer solutions for common problems in video microscopy.

Main Methods:

  • Identification of common errors in image quantitation.
  • Focus on practical aspects of video microscopy.
  • Guidance on avoiding pitfalls in image analysis.

Related Experiment Videos

Main Results:

  • Common errors include aspect ratio issues, automatic camera settings, improper dynamic range, and integer arithmetic.
  • Awareness of these issues is key to successful quantitation.
  • Technical manuals and industry support are valuable resources.

Conclusions:

  • Implementing practical strategies can significantly improve image quantitation accuracy.
  • Addressing specific technical challenges leads to more reliable scientific imaging.
  • Collaboration between academia and industry enhances video microscopy applications.