Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ratio imaging instrumentation

K Dunn1, F R Maxfield

  • 1Department of Medicine, Indiana University Medical Center, Indianapolis 46202-5116, USA.

Methods in Cell Biology
|March 21, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Perceptions of anonymised data use and awareness of the NHS data opt-out amongst patients, carers and healthcare staff.

Research involvement and engagement·2021
Same author

Mental ill-health in mothers of people with intellectual disabilities compared with mothers of typically developing people: a systematic review and meta-analysis.

Journal of intellectual disability research : JIDR·2021
Same author

Preventing transmission of bloodborne viruses from infected healthcare workers to patients: Summary of a new Canadian Guideline.

Canada communicable disease report = Releve des maladies transmissibles au Canada·2020
Same author

Infection prevention in personal services settings: Evidence, gaps and the way forward.

Canada communicable disease report = Releve des maladies transmissibles au Canada·2019
Same author

The National Advisory Committee on Infection Prevention and Control (NAC-IPC).

Canada communicable disease report = Releve des maladies transmissibles au Canada·2019
Same author

Separation of root nodule cells and identification of tissue-specific genes.

Plant cell reports·2019
Same journal

Quantification of cell viability by automated analysis of live cell imaging.

Methods in cell biology·2026
Same journal

Flow cytometry evaluation of cytotoxicity exerted by effector immune cells against tumor cells.

Methods in cell biology·2026
Same journal

Time-lapse confocal laser scanning microscopy analysis of FOOD formation.

Methods in cell biology·2026
Same journal

Screening and identification of protein-protein interaction using proximity labeling.

Methods in cell biology·2026
Same journal

Quantitative high-content profiling of mitochondrial morphology with automated statistical analysis and integrated data visualization.

Methods in cell biology·2026
Same journal

Super-resolution imaging of cell death in Drosophila tissues via expansion and pan-expansion microscopy.

Methods in cell biology·2026
See all related articles

Ratio imaging offers quantitative insights from microscope images but requires careful experimental design to overcome potential challenges. Advances in instrumentation, like improved CCD cameras and confocal microscopy, are enhancing its accuracy and applicability in cell biology.

Area of Science:

  • Cell Biology
  • Microscopy Techniques

Background:

  • Ratio imaging is a powerful technique for quantitative analysis of microscope images.
  • While effective, it is susceptible to various interfering parameters and potential measurement inaccuracies.

Purpose of the Study:

  • To discuss potential problems and considerations in designing ratio-imaging experiments.
  • To highlight advancements in instrumentation that improve ratio-imaging accuracy and applicability.

Main Methods:

  • Review of potential pitfalls in ratio-imaging experimental design.
  • Discussion of instrumentation advancements, including CCD cameras and confocal microscopy.
  • Consideration of axial chromatic aberration and Acousto-Optic Tunable Filters (AOTFs).

Main Results:

Related Experiment Videos

  • Ratio imaging, though not a panacea, reduces many interfering parameters.
  • Newer instrumentation, such as high-bit-depth CCD cameras and advanced confocal microscopes, improves quantitative measurements.
  • Developments address issues like axial chromatic aberration and enable wider applications through tools like AOTFs.

Conclusions:

  • Careful experimental design and investigator vigilance are crucial for accurate ratio-imaging measurements.
  • Instrumentation progress is continuously overcoming challenges, expanding the utility of ratio imaging.
  • Ratio imaging has broad applications in cell biology beyond ion concentration measurements, offering novel cellular insights.