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Single-strand conformation polymorphism (SSCP) analysis of Listeria monocytogenes iap gene as tool to detect
M Manzano1, L Cocolin, C Pipan
1Dipartimento di Scienze degli Alimenti, Facoltà di Agraria, Università di Udine, Italy.
Molecular and Cellular Probes
|March 17, 1998
Abstract:
PCR-single-strand conformation polymorphism (PCR-SSCP) analysis is a convenient technique for the detection of mutations. As the mobility of single-stranded DNA is sequence-dependent it could therefore be used to determine serotype-related sequence variations in Listeria monocytogenes. Sero-specific patterns were observed in different L. monocytogenes serogroups.