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Quantitative investigation of dipole-CSA cross-correlated relaxation by ZQ/DQ spectroscopy
B Brutscher1, N R Skrynnikov, T Bremi
1Laboratorium für Physikalische Chemie, ETH Zentrum, Zürich, 8092, Switzerland.
Abstract:
A zero-quantum/double-quantum HNCO(H) constant time experiment is presented for the quantitative evaluation of dipole-CSA cross-correlated relaxation involving the 1HN, 15N, and 13C' nuclei of the peptide plane. A simple procedure that allows the extraction of cross-correlated relaxation rate constants from intensity ratios of well-resolved doublet components along omega1 is described. The experiment is demonstrated on fully 13C, 15N-labeled ubiquitin.