Related Experiment Video
Updated: May 16, 2026

Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR
Published on: December 16, 2013
Accurate and precise measurement of heteronuclear long-range couplings by a gradient-enhanced two-dimensional
1Department of Chemistry, Georgia State University, Atlanta, Georgia, 30303-3083, USA.
Abstract:
We propose a phase-sensitive gradient-enhanced two-dimensional heteronuclear multiple-bond correlation (psge-2D HMBC) experiment for speedy, accurate, and precise measurement of 2JCH and 3JCH. The experiment does not suppress one-bond correlations. Rather, the value of a desired long-range JCH is obtained from the pertinent cross-peak pattern in the HMBC spectrum, using the corresponding 1JCH correlation pattern as reference. The application of the proposed experiment is illustrated for the trisaccharide raffinose.
More Related Videos
Related Concept Videos
¹H NMR: Interpreting Distorted and Overlapping Signals
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are slanted or...
2D NMR: Homonuclear Correlation Spectroscopy (COSY)
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)
¹H NMR: Long-Range Coupling
In alkenes, spin information is communicated via σ–π overlap, as seen in allylic (four-bond) and homoallylic (five-bond) couplings. These coupling interactions are stronger when the σ bond is parallel to the alkene π orbitals.
2D NMR: Overview of Homonuclear Correlation Techniques
COSY90 is the standard two-dimensional (2D) COSY experiment that...
2D NMR: Overview of Heteronuclear Correlation Techniques

