A gonad-derived survival signal for vulval precursor cells in two nematode species

Intercellular cell-survival signals play a major role in animal development [1]. In the nematode Caenorhabditis elegans, however, the stereotyped cell deaths that occur reproducibly during development are regulated in a cell-autonomous fashion (or, in a few cases, by a death-inducing signal) [2]. We show here the existence of a cell-survival signal acting on the vulval precursor cells in two nematodes, Turbatrix aceti and Halicephalobus sp. JB128. In C. elegans [3], as in many other nematode species [4] [5-7], ablation of the gonad causes all vulval precursor cells to adopt a default epidermal fate: a gonadal signal is required for the induction of vulval fates. In the nematodes T. aceti and Halicephalobus sp. JB128, however, we found that ablation of the gonad in the L1 larval stage caused all vulval precursor cells to undergo programed cell death. Thus, in intact Turbatrix and Halicephalobus, a survival signal from the gonad prevents activation of the cell-death program in vulval precursor cells. Our results demonstrate the existence of intercellular cell-survival signals in nematodes and uncover an evolutionary variation in the role of the gonad in nematode vulval development.