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Molecular cloning and characterization of a cDNA encoding asparagine synthetase from soybean (Glycine max L.) cell
H Yamagata1, A Nakajima, C Bowler
1Laboratory of Biochemistry, Faculty of Agriculture, Kobe University, Japan.
Bioscience, Biotechnology, and Biochemistry
|March 21, 1998
Abstract:
A cDNA encoding glutamine-dependent asparagine synthetase was isolated from dark-adapted Glycine max cell culture. The deduced amino acid sequence showed 76-89% identity with other plant sequences. The gene for asparagine synthetase is expressed predominantly in shoots as compared to roots of etiolated plants and the level of expression decreases following light treatment, suggesting that the gene expression is down-regulated by light.