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Purification of mouse interferon by sequential chromatography

I A Braude, E De Clercq

Journal of Chromatography

|April 21, 1979

Summary

Two purification schemes for mouse interferon (IFN) were developed using chromatography. These methods achieved high specific activity, yielding purified mouse IFN suitable for further research.

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Area of Science:

Biochemistry
Immunology
Protein Purification

Background:

Interferons (IFNs) are crucial cytokines with antiviral and immunomodulatory functions.
Efficient purification of mouse interferon (IFN) is essential for biochemical and functional studies.
Existing purification methods may lack the specificity or yield required for high-purity IFN.

Purpose of the Study:

To develop and compare two distinct chromatographic schemes for the purification of mouse interferon (IFN).
To achieve high specific activity of purified mouse IFN using a combination of physicochemical and affinity/adsorption techniques.

Main Methods:

Mouse IFN was purified using ammonium sulfate precipitation followed by various chromatographic techniques.
Scheme 1 involved hydrophobic chromatography (AFFI-Gel 202) and adsorption chromatography (Controlled Pore Glass).
Scheme 2 incorporated gel filtration (Ultrogel AcA 54), ion-exchange chromatography (Carboxymethyl Boi-Gel Agarose), hydrophobic chromatography (AFFI-Gel 202), and adsorption chromatography (Controlled Pore Glass).

Main Results:

Scheme 1 yielded mouse IFN with a final specific activity of 1.0–8.0 x 10^8 units/mg.
Scheme 2 resulted in mouse IFN with a final specific activity of 2.5–3.7 x 10^8 units/mg.
Both schemes demonstrated the effectiveness of combining different chromatographic principles for IFN purification.

Conclusions:

The described chromatographic schemes provide efficient methods for purifying mouse interferon (IFN) to high specific activity.
These purification strategies are valuable for obtaining research-grade mouse IFN for various biological applications.
The choice of chromatographic techniques can be tailored to optimize yield and purity based on specific research needs.

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