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Related Experiment Videos

Patterned acquisition of skin barrier function during development

M J Hardman1, P Sisi, D N Banbury

  • 1School of Biological Sciences, University of Manchester, Manchester M13 9PT, UK.

Development (Cambridge, England)
|June 20, 1998
PubMed
Summary
This summary is machine-generated.

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Embryonic skin barrier formation occurs in a patterned manner, starting at specific sites and spreading across the epidermis. This developmental process is crucial for survival in a terrestrial environment after birth.

Area of Science:

  • Developmental Biology
  • Epidermal Biology
  • Skin Barrier Physiology

Background:

  • The stratum corneum, the epidermis's outer layer, provides essential skin barrier function for terrestrial life.
  • Barrier formation is a late embryonic event, enabling fetal survival outside the womb.
  • Previous studies utilized trans-epidermal water loss (TEWL) assays for quantitative barrier assessment.

Purpose of the Study:

  • To investigate the spatial and temporal dynamics of skin barrier formation during embryonic development.
  • To characterize the cellular changes accompanying barrier acquisition.
  • To validate novel in situ permeability assays for studying embryonic skin development.

Main Methods:

  • Qualitative in situ assays for skin permeability on whole embryos.

Related Experiment Videos

  • Observation of epidermal changes in stratum corneum precursor cells.
  • Validation of assays using maternal glucocorticoid therapy and cross-species comparisons (mouse and rat).

    • Main Results:

    • Embryonic skin barrier forms in a patterned manner, initiating at specific epidermal sites and progressing as a moving front.
    • The barrier formation front is associated with significant cellular alterations in outer epidermal cells.
    • Final stages of cornified envelope assembly are synchronized with the onset of barrier function.
    • Patterned barrier formation was observed in rats, suggesting a conserved developmental mode across species.

    Conclusions:

    • In situ permeability assays effectively capture the developmental acquisition of skin barrier function.
    • Patterned development is a critical mode of epidermal maturation during embryogenesis.
    • The findings highlight the importance of developmental patterning for establishing essential physiological functions like the skin barrier.