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Competitive Genomic Screens of Barcoded Yeast Libraries
Published on: August 11, 2011
Zebrafish genomic library in yeast artificial chromosomes
T P Zhong1, K Kaphingst, U Akella
1Cardiovascular Research Center, Massachusetts General Hospital, Charlestown 02129, USA.
Genomics
|March 21, 1998
Summary
A new zebrafish yeast artificial chromosome (YAC) library was created, offering 4.7x genome coverage with 17,000 clones. This resource enables efficient screening for genetic research.
Area of Science:
- Genomics
- Molecular Biology
- Zebrafish Research
Background:
- The zebrafish (Danio rerio) is a vital model organism in genetic and developmental studies.
- Access to comprehensive genomic resources is crucial for advancing zebrafish research.
Purpose of the Study:
- To construct a high-quality yeast artificial chromosome (YAC) library for the zebrafish genome.
- To provide a scalable resource for genetic mapping and positional cloning in zebrafish.
Main Methods:
- Genomic DNA was isolated from the inbred AB zebrafish strain.
- A YAC library was constructed with an average insert size of 470 kb.
- The library comprises 17,000 clones, achieving approximately 4.7-fold genome coverage.
Main Results:
- The library provides extensive coverage of the zebrafish genome.
- YAC clones are arrayed for efficient screening via polymerase chain reaction (PCR).
- YAC ends are readily recoverable and sequenceable using mini-YAC clones.
Conclusions:
- The developed YAC library is a valuable resource for zebrafish genomics.
- This library facilitates high-throughput genetic analysis and gene discovery.
- The methodology allows for efficient characterization of YAC clones and their ends.
