Related Experiment Videos
Expression of the cDNA and purification of P0 ribosomal protein from Lupinus luteus
K Mikołajczyk1, J Barciszewski
1Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland.
Biochemistry and Molecular Biology International
|March 21, 1998
Abstract:
Eukaryotic ribosomal protein P0 is localized on the large ribosomal subunit at the base of the specific "stalk" structure, close to the central protuberance. Recently we have obtained a clone coding for P0 ribosomal protein from L. luteus (yellow lupin) cDNA library. Here we present its expression in E. coli cells and purification of the target protein.